m6A甲基化及预测方法工具总结

既然m6A甲基化具有这么重要的功能，且现在研究非常火爆，那我们是否可以预测RNA中的哪些位点会发生潜在的m6A甲基化呢，然后再决定是否有必要继续做相应的实验来验证呢。答案是肯定的！接下来，我们就讲一下有哪些软件或在线工具可以直接根据序列就预测RNA中潜在的m6A甲基化位点。

1. iRNA-Methyl

软件地址：http://lin.uestc.edu.cn/server/iRNA-Methyl

发表文章：Chen et al. iRNA-Methyl: Identifying N(6)-methyladenosine sites using pseudo nucleotide composition. Analytical Biochemistry，2015

2. SRAMP

软件地址：http://www.cuilab.cn/sramp/

发表文章：Zhou et al. SRAMP: prediction of mammalian N6-methyladenosine (m6A) sites based on sequence-derived features. Nucleic Acids Research, 2016

3. iRNAm5C-PseDNC

软件地址：http://www.jci-bioinfo.cn/iRNAm5C-PseDNC

发表文章：Qiu et al. iRNAm5C-PseDNC: identifying RNA 5-methylcytosine sites by incorporating physical-chemical properties into pseudo dinucleotide composition. Oncotarget, 2017

4. M6AMRFS

软件地址：http://server.malab.cn/M6AMRFS/

发表文章：Qiang et al. M6AMRFS: Robust Prediction of N6-Methyladenosine Sites With Sequence-Based Features in Multiple Species. Frontiers in Genetics, 2018

5. M6APred-EL

软件地址：http://server.malab.cn/M6APred-EL/

发表文章：Wei et al. M6APred-EL: A Sequence-Based Predictor for Identifying N6-methyladenosine Sites Using Ensemble Learning. Molecular Therapy: Nucleic Acids, 2018

6. DeepM6ASeq

软件地址：https://github.com/rreybeyb/DeepM6ASeq

发表文章：Zhang et al. DeepM6ASeq: prediction and characterization of m6A-containing sequences using deep learning. BMC Genomics, 2018

上面的这些软件可以预测RNA中的m6A甲基化位点，那么如何进一步注释m6A甲基化的功能呢体可用如下工具：

软件名称：m6Acomet

软件地址：http://www.xjtlu.edu.cn/biologicalsciences/m6acomet

发表文章：Wu et al. m6Acomet: large-scale functional prediction of individual m6 A RNA methylation sites from an RNA comethylation network. BMC Bioinformatics, 2019

希望今天的内容对大家有用，会持续更新经典内容，欢迎留言~~

参考文献

1. Fu et al. Gene expression regulation mediated through reversible mRNA methylation, Nature Reviews Genetics, 2014

2. Roundtree et al. Dynamic RNA Modifications in Gene Expression Regulation, Cell, 2017

3. Lee et al. Emerging Roles of RNA Modification: m6 A and U-Tail, Cell, 2017