生物分子解卷积和 告软件

ProMass是一种自动生物分子解卷积和 告软件包，用于处理Thermo Scientific的Xcalibur或Waters MassLynx数据系统上采集的ESI / LC / MS数据或单个ESI质谱图。ProMass被设计用于高通量应用。

当用电喷雾（ESI）离子化时，大的生物分子主要是多电荷的。当以正离子模式分析时，由于碱性氨基酸和氨基末端，肽或蛋白质将在多个位点质子化。另一方面，在负离子模式下分析的寡核苷酸由于酸性磷酸骨干而在多个位点去质子化。这种多次充电通常导致包含一系列电荷状态的ESI质谱。这些复杂的光谱必须“解卷积”或“转化”，以便确定生物分子的不带电荷的质量。 ProMass为执行整个LC / MS数据集的解卷积提供了一个自动化平台。

ProMass从一开始就设计用于高通量应用。实际上，运行ProMass的正常方式是通过Xcalibur或MassLynx示例列表。因此，您可以运行样品列表，让ProMass在处理它们，或者在您方便的时候批量重新处理列表。

ProMass使用称为ZNova的新型去卷积算法，可产生无伪像的解卷积质谱图。 ZNova可用于处理各种生物分子（包括蛋白质，寡核苷酸，肽等）的数据。与其他许多反卷积算法不同，ZNova可以适应低电荷状态谱和低信噪比的数据。 ProMass自动地以任何 页浏览器的易用和交互式格式 告结果。顶级摘要页面汇总了多次运行的结果，并将其显示在单个超链接页面上，以便您快速浏览结果。目标质量特征允许您自动从LC / MS数据中搜索多个目标质量数。目标质量特征包括结果颜色编码，使您可以快速磨合可能需要进一步关注的样品。

ProMass HR通过合并来自Positive Probability Ltd（PPL）的全套算法，包括用于高分辨率数据处理的其他功能。Promass HR方法可以利用PPL算法，该算法允许对同位素分辨的质谱进行单同位素质量测定或对同位素未分辨的质谱进行更高的分辨率。通过与Promass标准版本相同的强大机制，可以自动实现PPL方法。结果也通过相同的易于使用和直观的基于Wed的输出显示。

特征：

ProMass不仅仅是解卷积软件。ProMass是一个工具，允许您以高度协作的基于Web的格式处理和传达您的结果。

ProMass版本分为标准版和HR版本。ProMass HR版本包含标准版的所有功能，另外，HR版本还具备以下额外功能：

ProMass HR功能

ProMass for Xcalibur系统要求

用户应该遵循安装计划使用的Xcalibur版本的一般系统要求。运行ProMass for Xcalibur的系统要求如下：

注意： ProMass不包含Web服务器软件。如果要在本地计算机上查看结果，则不需要Web服务器。但是，如果您想通过Internet或Intranet与他人共享数据，则需要Web服务器。

英文简介

Introduction:

Large biomolecules when ionized by electrospray (ESI) are predominantly multiply charged. When analyzed in the positive ion mode, peptides or proteins will be protonated at multiple sites as a result of the basic amino acids and amino terminus. On the other hand, oligonucleotides analyzed in the negative ion mode are de-protonated at multiple sites as a result of the acidic phosphate backbone. This multiple charging usually results in ESI mass spectra that contain a range of charge states. These complex spectra must be “deconvoluted” or “transformed” in order to order to determine the uncharged mass of the biomolecule. ProMass provides an automated platform for performing deconvolution of entire LC/MS data sets.

Overview:

ProMass is an automated biomolecule deconvolution and reporting software package that is used to process ESI/LC/MS data or single ESI mass spectra acquired on Thermo Scientific’s Xcalibur or Waters MassLynx data systems. ProMass was designed from the start for high-throughput applications. In fact, the normal way to run ProMass is through the Xcalibur or MassLynx sample list. Therefore, you can run a list of samples and have ProMass process them at the end or batch reprocess the list at your convenience.

ProMass uses a novel deconvolution algorithm known as ZNova that produces artifact-free deconvoluted mass spectra. ZNova can be used to process data from a wide variety of biomolecules including proteins, oligonucleotides, peptides, etc. Unlike many other deconvolution algorithms, ZNova can accommodate low charge state spectra and data of low signal-to-noise ratio. ProMass reports the results automatically in an easy-to-use and interactive format viewable from any web browser. A top-level summary page summarizes the results from multiple runs and displays them on a single hyperlinked page allowing you to navigate your results quickly. Target mass features allow you to search for multiple target masses from LC/MS data automatically. The target mass features include results color coding, allowing you to quickly hone in on the samples that may require further attention.

ProMass HR includes additional features for high-resolution data processing by incorporating the full suite of algorithms from Positive Probability Ltd (PPL). ProMass HR methods can utilize the PPL algorithms that allow for monoisotopic mass determination of isotopically resolved mass spectra or enhanced resolution of isotopically unresolved mass spectra. The PPL methods may be automated thru the same powerful mechanism as the standard version of ProMass. Results are displayed through the same easy-to-use and intuitive web-based output as well.

Key Features of ProMass:

ProMass is more than just deconvolution software. ProMass is a tool that allows you to process and communicate your results in a highly collaborative web-based format.

Additional Features of ProMass HR:

ProMass HR includes all of the features of the standard version of ProMass, plus the following:

ProMass for Xcalibur System Requirements

You should follow the general system requirements for installing the version of Xcalibur that you plan to use. The system requirements for running ProMass for Xcalibur are listed below:

Spectral deconvolution is a processor-intense operation. Therefore, a fast microprocessor is best if you want fast execution times or if you are processing complex spectra.

Note:ProMass does not include web server software. A web server is not required if you want to view your results on a local machine. However, if you want to share your data with others over the internet or intranet you will need a web server.

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